Ahmad Fahmi

Sporosarcina pasteurii is one of the soil inhabitant and urease-producing microorganisms fromwhich urease enzyme is made, causing bio-calcification. In this research, optimizing of urease enzymeproduced by Sporosarcina pasteurii bacteria was targeted to connect the sand particles and stiffen them bythe production of calcite precipitation. Bacterium was cultivated aerobically in a culture medium containingyeast extract, lacto pepton and urea at the temperature of 25°C. Different conditions of cultivation with Ureaconcentration calcium chloride and pH were examined to obtain optimal results. The optimum conditionfor the stiffening of sand grains was attained in the presence of urea (20 g/L), calcium chloride (30mM) andthe pH of 9. The results of this research indicated that the sand calcification via urease enzyme producedby Sporosarcina pasteurii could be employed for the hardening of the sand provided that the efficient factorsof calcite precipitation could be optimized.