Abstract: Escherichia coli (E. coli) bacteria is one of the hazardous human pathogens. Consequently,
developing the rapid and effective method for identification and quantization of E. coli is popular in
biotechnological researches in recent years.
Experimental: In this research, a label-free capacitance E. coli biosensor was fabricated based on immobilizing
bacteriophage on the carbon paste electrode (Cp). Reduced graphene (RGr) was synthesized
and used as a substrate for immobilization of bacteriophage on the Cp surface. E. coli bacteriophage
was trapped in graphene modified carbon paste electrodes. The immobilization accuracy was confirmed
via electrochemical techniques. The modified electrodes were applied as indicator electrodes for capacitance
measurements of E. coli.
Conclusion: Through this method, E. coli was detected in a concentration range of 33×10-3 to 330×10-3
N L-1 (number of E. coli per Liter) with a correlation coefficient of 0.99 and a detection limit of 12×10-3
N L-1. The proposed biosensor has a fast response time of about 5 s and good selectivity over other bacteria.