زهرا سیار

Curcumin is a natural and non-polar pigment with unique properties such as antioxidant activity that has been widely utilized in food and pharmaceutical formulations. In this study, solvent displacement and subcritical water methods were used to prepare Curcumin nanodispersions. The influences of the method and storage temperature were evaluated on physical and chemical stability of the prepared nanodispersions. Obtained results indicated that prepared transparent nanodispersions maintained their physical and chemical stability at 4 and 25 °C for 4 months. The results also showed that the prepared nanodispersions using subcritical water (temperature of 120 °C and pressure of 1.5 atm for 2 h) had a higher stability (minimum changes in the particle size) and less Curcumin loss (0.36%). Release of Curcumin from prepared nanodispersions was also evaluated at different pH values (1.2, 5.4, and 7.4) and the results indicated that its release rate was higher in pH values ranging 5 to 7. Kinetic study showed that release of Curcumin from dialysis bag was controlled by diffusion at pH value of 7.4 with a high constant rate. The viability percentage of HT-29 cells against pure Curcumin and Curcumin nanodispersions prepared using solvent displacement and subcritical water methods for 72 h incubation were 94, 30 and 18%, respectively. Moreover, In drug delivery systems, mathematical modeling and simulation play an important role in elucidating important drug release mechanisms; therefore, the results of experimental data of diffusion rate were compared with those obtained from simulation, and a good agreement was foun