Biomedical Materials Purpose-led Publishing, find out more. PAPER Co electrospinning -poly (vinyl alcohol)-chitosan/gelatin-poly (epsilon-caprolacton) nanofibers for diabetic wound-healing application Marziyeh Ranjbar-Mohammadi4,1, Farideh Tajdar1, Elnaz Esmizadeh2 and Zahra Arab3 Published 30 May 2024 • © 2024 IOP Publishing Ltd Biomedical Materials, Volume 19, Number 4 Citation Marziyeh Ranjbar-Mohammadi et al 2024 Biomed. Mater. 19 045017 DOI 10.1088/1748-605X/ad4df6 References Open science Article metrics 42 Total downloads Submit Submit to this Journal Permissions Get permission to re-use this article Share this article Article and author information Abstract With the increasing prevalence of diabetes, the healing of diabetic wounds has become a significant challenge for both healthcare professionals and patients. Recognizing the urgent need for effective solutions, it is crucial to develop suitable scaffolds specifically tailored for diabetic wound healing. In line with this objective, we have developed novel hybrid nanofibrous scaffolds by combining polyvinyl alcohol/chitosan (PVA/CS) and gelatin/poly(ε-caprolactone) (Gel/PCL) polymers through a double-nozzle electrospinning technique. In this study, we investigated the influence of the Gel/PCL blend ratio on the properties of the resulting nanofibers. Three different hybrid scaffold structures were examined: Gel/PCL (80:20)-PVA/CS (80:20), Gel/PCL (50:50)-PVA/CS (80:20), and Gel/PVA (20:80)-PVA/CS (80:20). Our findings demonstrate that the electrospun nanofibers of PVA/CS (80:20)-Gel/PCL (80:20) exhibited optimal mechanical performance, with a contact angle of approximately 54° and a diameter of 183 nm. Considering the crucial role of inhibiting bacterial adhesion in the success of implanted materials, we evaluated the cytocompatibility of the hybrid electrospun nanofibers using mouse fibroblast cells (L-929 cells). The in vitro cytotoxicity results obtained from L-929 fibroblast cell culture on the hybrid scaffolds revealed enhanced cell proliferation and appropriate cell morphology on the PVA/CS (80:20)-Gel/PCL (80:20) sample, indicating its capability to support tissue cell integration. Based on the information obtained from this study, the fabricated hybrid scaffold holds great promise for diabetic ulcer healing. Its optimal mechanical properties, suitable contact angle, and favorable cytocompatibility highlight its potential as a valuable tool in the field of diabetic wound healing. The development of such hybrid scaffolds represents a significant step forward in addressing the challenges associated with diabetic wound care.
