In this investigation, nanofibrous scaffold was fabricated through electrospinning of
Polycaprolactone (PCL) and Chitosan (CS) using novel collector to make better
orientation and pore size for cell infiltration. PCL/CS nanofibers with 90-rpm collector
speed and 40- degree angle between collector wires of new collector has fewer diameters
with better pore size and nanofibers orientation. Mechanical properties, roughness
parameters, topology structure, hydrophilicity and cell growing was considered for liver
tissue engineering. Cell culture was done using epithelial liver mouse cells. The
developed electrospun PCL/CS scaffold using novel collector would be an excellent
matrix for biomedical applications especially liver tissue engineering.