مرضیه رنجبر محمدی

Aim: This study investigated the potential application of a nanofibrous structure mimicking the natural extracellular matrix (ECM) of the body, composed of keratin/polyvinyl alcohol (K/PVA), polylactic acid (PLA), and chitosan nanofibrils/zinc oxide nanoparticles (ZnONPs/CHNF) (CSZ). Methods: In this research, hybrid nanofibrous scaffolds were produced using K/PVA and PLA via the electrospinning method. K/PVA from one syringe and PLA from another were injected to produce hybrid nanofibers. Nanofibrillated chitosan/zinc oxide nanoparticles (ZnONPs/CHNF) (CSZ) at three different blend ratios (1:0, 2:1, and 1:2) were incorporated into the polymeric solutions containing K/PVA. The hydrophilicity of the samples was evaluated by measuring the contact angle, and the morphology of the produced scaffolds was examined using a scanning electron microscope (SEM). The effect of CSZ on morphology, hydrophilicity, and cell culture properties was then assessed. Findings: The results demonstrated that the addition of ZnONPs to the CSZ solution led to a reduction in nanofiber diameter and an increase in the contact angle of the produced scaffolds. Extensive and suitable fibroblast cell growth was observed on the (2:1) PLA/CSZ-(2:1) K/PVA/CSZ sample. This sample, with a diameter of 352.50 ± 31 nm and a contact angle of 47 ± 3°, closely mimics the structure of the body's extracellular matrix (ECM). Its high surface-to-volume ratio enhances cell adhesion, proliferation, and growth, identifying it as the optimal sample.