Xanthene and its derivatives are important in medical diagnosis and laser technology. In this work, spectral features of fluorescein disodium were investigated in different environments with different polarity characteristics. Hence, the absorption and fluorescence spectra of this molecule were studied in different environments. Our results show that spectral features of fluorescein disodium depend strongly on substituents in its structure and molecular interactions. To investigate the contribution of various molecular interactions on the spectral behavior of the used sample, the linear solvation energy relationship concept is used. Moreover, the dipole moment variations from ground to excited states were estimated using molecular spectral features in different solvent media. According to the results, the dipole moment value in the excited state is higher than that in the ground state.
