Miniaturization has been widely applied in biosensing and immunosensing applications nowadays. Due to low Reynolds number flow, microchips usually suffer from mass transport and low detection yield. It should be noted that the antibody is immobilized on the surface of the biosensor, and the antigen is in the mobile phase. In this research, we propose an electrode structure to generate rotational flow near the biosensor and enhance the antibody-antigen binding process on the surface of the immune-sensor. Electrodes are excited at a proper voltage and frequency to generate an AC Electrothermal flow effect near the biosensor and concentrate the antigen to the immobilized antibodies. To study the AC electrothermal effect for an immunsensor, the physical equations, including electrostatic, fluid mechanics, temperature field, species concentration, and antibody-antigen binding reaction, is solved by the finite element method. By generating the electrothermal flow, the best location for the immunosensor is determined. The binding reaction is investigated both with and without applying the AC electrothermal effect. Based on the results with application of electrothermal flow (12-volt, 500 kHz), a rotational flow is induced and leads to a 9-fold improvement in antibody-antigen binding. The proposed system is studied for different Damköhler and Peclet numbers, and high throughput binding enhancement is observed for miniaturized and on-chip immunosensors
