In the present study, fructose molecules were employed both as a fuel and a bioactive agent within a green synthesis approach to prepare cluster-like Ag2WO4 nanoparticles (CL-Ag2WO4 NPs). A novel sensor for ciprofloxacin (CFX) determination was fabricated by modifying a screen-printed electrode (SPE) with the synthesized CL-Ag2WO4 nanoparticles (CL-Ag2WO4/SPE). The key electrochemical characteristics of the CL-Ag2WO4/SPE for CFX detection were evaluated using cyclic voltammetry (CV), chronoamperometry, and differential pulse voltammetry (DPV) techniques. Notably, the CL-Ag2WO4/SPE demonstrated superior electrical conductivity and excellent electrochemical performance for the electrooxidation of CFX in a neutral medium compared to the bare SPE. The developed sensor exhibited a broad linear detection range of 0.05–130 µM, with a low detection limit (LOD) of 0.047 µM. Additionally, the CL-Ag2WO4/SPE enabled the determination of ciprofloxacin in the presence of metoclopramide (MET), with well-separated oxidation peak potentials. The practicality and reliability of the fabricated sensor for CFX and MET detection were further validated in real samples. The CL-Ag2WO4/SPE electrochemical sensor offered a simple, rapid, sensitive, and cost-effective solution, presenting a promising strategy for the accurate quantification of CFX in urine and tablet samples.
