Elham Naghashzargar

In this investigation, nanofibrous scaffold was fabricated through electrospinning of Polycaprolactone (PCL) and Chitosan (CS) using novel collector to make better orientation and pore size for cell infiltration. PCL/CS nanofibers with 90-rpm collector speed and 40- degree angle between collector wires of new collector has fewer diameters with better pore size and nanofibers orientation. Mechanical properties, roughness parameters, topology structure, hydrophilicity and cell growing was considered for liver tissue engineering. Cell culture was done using epithelial liver mouse cells. The developed electrospun PCL/CS scaffold using novel collector would be an excellent matrix for biomedical applications especially liver tissue engineering.