کلیدواژهها
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Drug delivery system, Alginate, Nanoclay, PH-sensitive nanocomposite, Cancer therapy, Antibacterial
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چکیده
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Purpose: In last decades, by increasing multi-drug resistant microbial pathogens an urgent
demand was felt in the development of novel antimicrobial agents.
Methods: Promising nanocomposites composed of clay/alginate/imidazolium-based ionic
liquid, have been developed via intercalation of calcium alginate and ionic liquid by ionexchange
method. These tailored nanocomposites were used as nanocarriers to simultaneously
deliver methotrexate (MTX), and ciprofloxacin (CIP), as anticancer and antibacterial agents,
respectively to MCF-7 breast cancer cells. Nanocomposites were fully characterized by
scanning electron microscopy studies (SEM), X-ray diffraction (XRD), Fourier transforms infrared
(FTIR) spectroscopy, and thermogravimetric analysis (TGA) methods. The in vitro antimicrobial
potential of the mentioned nanocomposites in free and dual-drug loaded form was investigated
on Pseudomonas aeruginosa and Escherichia coli bacteria. The antitumor activity of nanoformulations
was evaluated by both MTT assay and cell cycle arrest.
Results: The dual drug-loaded nanocomposites with exceptionally high loading efficiency
(MTX: 99 ±0.4% and CIP: 98 ±1.2%) and mean particle size of 70 nm were obtained with
obvious pH-responsive MTX and CIP release (both drugs release rate was increased at pH 5.8
compared to 7.4). The antibacterial activity of CIP-loaded nanocomposites was significantly
higher in comparison with free CIP (P < 0.001). The antitumor activity results revealed that
MTX cytotoxicity on MCF-7 cells was significantly higher in nano-formulations compared to
free MTX (P < 0.001). Both MTX-loaded nanocomposites caused S-phase arrest in MCF-7 cells
compared to non-treated cells (P ˂ 0.001).
Conclusion: Newly developed smart nanocomposites are potentially effective pH-sustainable
delivery systems for enhanced tumor therapy.
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